Women, compared to men, will evidence greater levels of perceived threat and loss of psychological resources, with resulting higher levels of negative affect and lower levels of positive affect. Each point of its space a reference frame becomes a whole three-dimensional space, and each point of this internal space is supposed to be somehow structured too, when it comes to accounting for spin and other intrinsic symmetries. Here we present a trial of biotin production using the food yeast Candida utilis. Psychological experiments differ by the degree of observer involvement, and the same formal techniques are available, save that one is to avoid any destructive methods. Os resultados obtidos podem ser sumarizados como:
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Biomass cell in the cultures supplemented with selenium ranged from 1. This plan was not a coup but only a general insurrection. Hence, the debate on which physics is more appropriate for describing consciousness quantum or classical mechanics, relativistic or nonrelativistic theory, etc is meaningless; no kind of physics describes psychological phenomena, while all kinds of physics can equally be made paradigms for psychological study. Esterification renders meso- and deutero-porphyrins inactive as substrates. Therefore this article problematizes urbanization as not only a process formed by the accumulation of capital and its distribution, but also a cultural process which requires the consent of social actors to such formation.
To provide scientific basis for the utilization and development of Mucuna pruriens var. Moisture, ash and the extracts of Mucuna pruriens var. The TLC spots of levodopa had similar color with the control group at the same position.
The results of HPLC quantitative analysis showed that the linear range of levodopa was This method is convenient, accurate, reliable with good reproducibility, so it can be used to establish quality standard for the medicinal material.
Recombinant Candida utilis for the production of biotin. Biotin is an important nutritional supplement but is difficult to manufacture effectively. Here we present a trial of biotin production using the food yeast Candida utilis.
In this system, we cloned the C.
The BIO2 gene can be successfully integrated into the Mdoifier. The biotin yield in this system can reach fold above the endogenous level in a small-scale culture.
Although the biotin production is not stable if the selection pressure is removed, this system has the potential to produce biotin-rich feed or food additives directly without the requirement of further purification. Export and accumulation of various forms of invertase EC 3. It was found that the high-molecular-weight CW-form of invertase is present in the cell wall.
This midifier is not exported into the culture liquid, and it is by a third more glycosylated than the previously described exported S-form. It was shown that one of the two liquid forms of invertase exported into the culture-the glycosylated S-form–is retained in the cell wall, while the other one–the nonglycosylated F-form–was not detected in the cell wall.
Based on these results, as well as data on the distribution dynamics of the enzyme in the culture liquid and in the cell wall during different growth stages of a yeast culture, we suggested that the nonglycosylated form was exported into the culture liquid subtiltes the zone of abnormal cell wall permeability and the glycosylated forms of this enzyme both exported and nonexported did not use this pathway the degree of N-glycosylation is an important factor determining the final localization of the enzyme.
Functional purification of the monocarboxylate transporter of the yeast Candida utilis. Plasma membranes of the yeast, Candida utiliswere solubilized with octyl-beta-D-glucopyranoside and a fraction enriched in the lactate carrier was obtained with DEAE-Sepharose anion-exchange chromatography, after elution with 0. The uptake of lactic acid into proteoliposomes, containing the purified protein fraction and cytochrome c oxidase, was dependent on a proton-motive force and the transport specificity was consistent with the one of C.
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Overall, we have obtained a plasma membrane fraction enriched in the lactate carrier of C. Given the similarities between the lactate transport of C. Candida utilis and Cyberlindnera Pichia jadinii: The yeast Candida utilis is used as a food additive and as a host for heterologous gene expression to produce various metabolites and proteins. Reliable protocols for intracellular production of recombinant proteins are available for C.
Whole genome sequencing of C. The review summarizes the status of recombinant protein production in C. Draft Genome Sequence of Staphylococcus carnosus subsp. Staphylococcus carnosus is used as a starter culture in meat fermentation, where it contributes to color formation and produces aromatic compounds.
Here, we report the first draft genome sequence of an S. Selenium is considered an essential component of all living organisms. modkfier
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The use of yeasts as a selenium supplement in human nutrition has gained much interest over the last decade. The accumulation and biochemical transformation of selenium in yeast cells is particularly interesting to many researchers. In this article, we present the results of the determination of selenium and selenomethionine subtitkes in the biomass of feed yeast Candida utilis ATCC obtained from the culture grown in a bioreactor.
The results indicated that C.
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The highest content of selenomethionine Biomass cell in the cultures supplemented with selenium ranged from 1. The results of this study indicate that yeast cell biomass of C. It creates the possibility of obtaining selenium biocomplexes that can be used in the production of protein-selenium moodifier supplements for animals and humans.
Production of Candida utilis on slop by-product of fermentation industries. The slop vinas liquor, a major by-product a alcohol fermentation industries, has been used as growth medium for production of the torula yeast, Candida utilis. Supplementation of the slop with 0. The crude slop gave better results than the diluted or centrifuged liquors. Under optimal conditions, more than 15 grams of yeast were obtained on dry weight basis. The application feasibility of these results is presented.
Arginine mediated purification of trehalosephosphate synthase TPS from Candida utilis: Its characterization and regulation.
Trehalose is the most important multifunctional, non-reducing disaccharide found in nature. It is synthesized in yeast by an enzyme complex: In the present study TPS is purified using a new methodology from Candida utilis cells by inclusion of mM l-arginine during cell lysis and in the mobile phase of high performance gel filtration liquid chromatography HPGFLC.
An electrophoretically homogenous TPS that was purified was a 60 kDa protein with Aggregation mediated enzyme regulation was subtitlws. Among mocifier four glucosyl donors, the highest V max and lowest K m values were calculated as 2.
Among the glucosyl acceptors, glucosephosphate GP showed maximum activity followed by fructosephosphate FP. Polyanions heparin and chondroitin sulfate were seen to stimulate TPS activity with different glucosyl donors. Substrate specificity, V max and K m values provided an insight into an altered trehalose metabolic pathway in the C.
The present work employs a new purification strategy as well as highlights an altered pathway in C. Antimicrobial activity of fractions and subfractions of Elaeagia utilis against microorganisms of importance in dental caries. Dental caries is a multifactorial infectious disease that leads to the destruction of dental hard tissue. The main goal of research into medicinal plants is to seek compounds with antimicrobial activity for subsequent use in prevention strategies and control of infectious diseases.
The aim of this study was to evaluate the antimicrobial activity of fractions and subfractions obtained from Elaeagia utilis against Streptococcus mutans, Streptococcus sobrinus and Lactobacillus acidophilus. The plant material was collected in the town of Alban Cundinamarca, Colombiawhich is located at an altitude of meters above sea level. Two extracts were obtained by cold maceration of E. The antimicrobial activity of fractions and subfractions was evaluated by the well diffusion method.
Among the ethanol extract fractions, the dichloromethane fraction had notably greater antimicrobial activity. It was sub-partitioned, yielding three subfractions with inhibitory activity, of which the most active was MeOH: H2O Bp with minimum inhibitory concentration 0. Terpenes, sesquiterpenlactones and simple phenolic compounds were identified in it.
In conclusion, this study shows the antimicrobial potential of fractions and subfractions obtained from extracts of E. Biofiltration of volatile ethanol using sugar cane bagasse inoculated with Candida utilis.
Ethanol loads from At a loading rate of Ethanol removal was complete, and Acetaldehyde and ethyl acetate in the outlet gas attained 7. In both cases, the transient phase was less than one day. At a high inlet ethanol concentration In the three cases, final biomass was poor, ranging from Hyperaccumulation of zinc by zinc-depleted Candida utilis grown in chemostat culture. The steady-state levels of zinc in Candida utilis yeast grown in continuous culture under conditions of zinc limitations are utilis contains elevated amounts of polyphosphate and this anionic evidence does not allow discrimination between possible regulation of zinc homestasis either by inhibitions of zinc efflux through control of the membrane carrier or by control of the synthesis of a cytoplasmic zinc-sequestering macromolecule.
Protein enrichment of an Opuntia ficus-indica cladode hydrolysate by cultivation of Candida utilis and Kluyveromyces marxianus. We investigated protein enrichment by cultivation of the yeasts Candida utilis and Kluyveromyces marxianus in an enzymatic hydrolysate of the cladode biomass.
Yeast cultivation more than doubled the protein content of the hydrolysate, with an essential amino acid profile superior to sorghum moodifier millet grains. Despite its low biomass yield, its performance compared well with C. This is the first report showing that the protein content and quality of O.
The cladodes of Opuntia ficus-indica prickly pear cactus have a low protein content; for use as a balanced feed, supplementation with other subtitlrs sources is therefore desirable. Dilute acid pretreatment and enzymatic hydrolysis of sun-dried cladodes resulted in a hydrolysate containing per litre The modicier of dissolved oxygen DO and pH on glutathione batch fermentation by Candida utilis WSH in a 7 liters stirred fermentor were investigated.
It was shown that DO concentration is an important factor in glutathione production. Intracellular glutathione leakages were observed when the pH was 1. To assess the effect of pH on glutathione production, six batch processes controlled at pH 4. The yield was highest at pH 5. The maximal intracellular glutathione content 2. To improve our understandings on the effect of pH on the batch glutathione production, a modified Logistic equation and Luedeking-Piret equation were used to simulate cell growth and glutathione production, respectively, under different pH.
Based mdoifier the parameters obtained by the nonlinear estimation, kinetic analysis was performed to elucidate the effect of pH on the batch glutathione production. The process controlled at pH 5. Purification and characterization of a trehalase-invertase enzyme with dual activity from Candida utilis. Trehalose and sucrose, two important anti-stress non-reducing natural disaccharides, are catabolized by two enzymes, namely trehalase and invertase respectively.
In this study, a kDa enzyme protein active against both substrates was purified from wild type Candida utilis and characterized in detail.
Substrate specificity assay and activity staining revealed the enzyme to be specific for both sucrose and trehalose.